Van Eck, J., Keen, P., & Tjahjadi, M. (2018). Agrobacterium tumefaciens-mediated transformation of tomato. Methods in Molecular Biology, 225–234.
In this study, the authors focused on optimizing the tomato transformation protocol using Agrobacterium tumefaciens. To do this, they cultivated tomato plants under sterile conditions in vitro and subjected young cotyledon leaves to transformation. The authors transferred genetic constructs containing specific resistance genes to antibiotics and herbicides. Through selection using the agents described above, scientists identified transformed plants and concluded the most effective transformation protocols.
This article is helpful for the speech, as it contains information on the methods of Agrobacterium-mediated plant transformation. The article provides a detailed description of the process of cultivating crops under in vitro conditions, as well as the methods and mechanisms for obtaining transgenic plants. Additionally, this paper provides information on procedures for screening the results of genetic transformation and outlines the most effective protocols for this purpose.
Cunningham, F. J., Demirer, G. S., Goh, N. S., Zhang, H., & Landry, M. P. (2020). Nanobiolistics: An emerging genetic transformation approach. Methods in Molecular Biology, 141–159.
The gene gun, also known as biolistics, is a well-established and extensively studied method of plant genetic engineering. This method enables the transformation of not only the nuclear genome but also the plastid genome. The use of large metal particles can damage plant cells and be ineffective. The authors of this article explored the possibility of using nanoparticles as a more advanced version of biolistics, which would be less destructive to cells being transformed.
This source is valuable because it describes a classic genetic transformation method, such as the gene gun. The article provides a detailed description of the technology’s principle of operation, as well as its primary advantages and disadvantages. The paper also contains data that this method has modern modifications. To date, it is still relevant and can compete with other, more advanced techniques of plant genetic transformation.
Garland, S. (2020). Stop arguing over GMO crops. Scientific American.
The use of genetically modified plant crops is a topic of debate, with both supporters and opponents. Despite the public’s fear of GMO crops as unnatural and allegedly dangerous to health, such plants could solve many of humanity’s problems, including hunger. The article’s author considers this problem and the approach of the American authorities to its solution. The message conveyed by the paper is that people should be more accepting of genetically modified plants.
This source is valuable because it contains information about how global problems can be addressed by transgenic plants and reports on the challenges faced in distributing such crops. The paper describes approaches to introducing genetically modified crops that will benefit from them. This article provides insight into the benefits of plant genetic transformation and what steps the government is taking to promote this practice.
Little, A. (2022). We must learn to love genetically modified crops. Bloomberg.
The problem of world hunger today is becoming increasingly urgent due to climate change and the war unleashed by Russia against Ukraine. Transgenic crops are much more productive and stress-resistant than traditional plants. The article discusses how the use of genetically modified crops can help alleviate the problem of food shortages and save many human lives. The article provides specific examples of plant cultures that have undergone genetic modifications.
This source is important because it demonstrates the need for transgenic crops to address global issues such as hunger. The paper contains examples of specific bioengineering solutions that have improved the productivity and resilience of various crops. The article contains information that the agricultural industry is already using such crops. The author demonstrates how the wise use of modern technology can benefit humanity.
Raman, R. (2020). GMOs: Pros and Cons, Backed by Evidence. Healthline.
The article explains precisely what the term “genetically modified food” means, as well as its associated advantages and disadvantages. The author considers the primary health risks associated with the spread of genetically modified crops, including the risk of allergies and environmental risks related to the use of herbicides. The article also mentions the legislative regulation of GM products and their prevalence in stores.
The value of this source lies in its detailed coverage of the effects of transgenic crops on health and the environment. Key data include the safety of GMO food in relation to cancer risk. Moreover, the source examines the necessary legal and social aspects of genetically modified products, which aid in understanding the topic, and outlines the potential economic costs of utilizing these plants.